quantitative g6pd kit trinity g-6-pdh kit  (Trinity Biotech)

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Identification of G6PD Aures by an ARMS-PCR assay. a Schematic representation of the primer location and predicted size of PCR products for the G6PD Aures and internal control fragment. b Agarose gel electrophoresis represents band of G6PD Aures (226 bp) and internal control fragments (519 bp). Lane 1 represents the 100 bp DNA marker. Lanes 2–4 represent G6PD Aures-positive samples, and lanes 5–6, G6PD Aures-negative samples

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Control, Agarose Gel Electrophoresis, Marker

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Agarose gel electrophoresis representing 8 G6PD Asian genotypes. Lane 1 represents a 100 bp DNA marker. Lane 2 is a G6PD mutation standard marker. Lanes 3–7 are PCR products of unknown samples, lane 8 is the normal control, and lane 9 is the mutant positive control

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Agarose Gel Electrophoresis, Marker, Mutagenesis, Control, Positive Control

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Prevalence and frequency of G6PD gene mutations in the Lao Theung ethnic group

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques:

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: The histogram shows the correlation between G6PD activity (U/gHb) and G6PD mutations in the Lao Theung population. a G6PD activity (U/gHb) in each G6PD mutation group for males. Enzyme activity below 2.0 U/gHb indicates G6PD deficiency; enzyme activity of 2.0 to 4.67 U/gHb indicates G6PD intermediate. All hemizygous subjects presented G6PD deficiency. b G6PD activity for the female population. Homozygous subjects presented G6PD deficiency, while the heterozygous subjects have intermediate to normal G6PD enzyme activity

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Activity Assay, Mutagenesis

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Prevalence and molecular characteristics of G6PD deficiency in Lao PDR

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Mutagenesis

Journal: Malaria Journal

Article Title: Investigation of glucose-6-phosphate dehydrogenase (G6PD) deficiency prevalence in a Plasmodium vivax -endemic area in the Republic of Korea (ROK)

doi: 10.1186/s12936-020-03393-4

Figure Lengend Snippet: Schematic diagram of sample selection for enzymatic and genetic evaluation of G6PD deficiency. In accordance with IRB-approved (AFMC-17-IRB-023) protocol, blood samples were collected from soldiers who agreed to participate in the study. All 1632 blood samples were collected in two cities, Yangju (n = 853) and Paju (n = 779) and were screened using the CareStart G6PD and Hb POC test. Based on screened results, 134 samples that were below (30–80%; n = 131) and above (> 150%; n = 3) the G6PD median value underwent genetic analysis using a G6PD genotyping kit and full-length sequencing. Thirty-six normal-range G6PD samples were also tested. Paju recruitment training camp: n = 779/1007, consent rate = 77%; Yangju recruitment training camp: n = 853/1011, consent rate = 84%

Article Snippet: Fig. 2 Comparison of two analytical methods for representing G6PD activity: Point Scientific G-6-PDH kit and CareStart G6PD Biosensor.

Techniques: Selection, Sequencing

Journal: Malaria Journal

Article Title: Investigation of glucose-6-phosphate dehydrogenase (G6PD) deficiency prevalence in a Plasmodium vivax -endemic area in the Republic of Korea (ROK)

doi: 10.1186/s12936-020-03393-4

Figure Lengend Snippet: Primer schematic for G6PD gene sequencing. For further SNP evaluation of G6PD (from exon 3 to exon 13), nested PCR and sequencing primers were designed

Article Snippet: Fig. 2 Comparison of two analytical methods for representing G6PD activity: Point Scientific G-6-PDH kit and CareStart G6PD Biosensor.

Techniques: Sequencing, Nested PCR

Journal: Malaria Journal

Article Title: Investigation of glucose-6-phosphate dehydrogenase (G6PD) deficiency prevalence in a Plasmodium vivax -endemic area in the Republic of Korea (ROK)

doi: 10.1186/s12936-020-03393-4

Figure Lengend Snippet: Comparison of two analytical methods for representing G6PD activity: Point Scientific G-6-PDH kit and CareStart G6PD Biosensor. Using two analytical methods (Pointe Scientific G-6-PDH kit and CareStart G6PD Biosensor), G6PD activity from 3 representative samples are presented. Significance was calculated using an unpaired, two-tailed t-test (mean ± SEM; n = 3 for both the Point Scientific and the POCT Analyzer tests)

Article Snippet: Fig. 2 Comparison of two analytical methods for representing G6PD activity: Point Scientific G-6-PDH kit and CareStart G6PD Biosensor.

Techniques: Comparison, Activity Assay, Two Tailed Test

Journal: Malaria Journal

Article Title: Investigation of glucose-6-phosphate dehydrogenase (G6PD) deficiency prevalence in a Plasmodium vivax -endemic area in the Republic of Korea (ROK)

doi: 10.1186/s12936-020-03393-4

Figure Lengend Snippet: Distribution of G6PD activity. G6PD activity values for all 1632 participants fell within the normal range G6PD values (2.27–11.373 U/gHb), and the median value was 7.582 U/gHb

Article Snippet: Fig. 2 Comparison of two analytical methods for representing G6PD activity: Point Scientific G-6-PDH kit and CareStart G6PD Biosensor.

Techniques: Activity Assay

Journal: Malaria Journal

Article Title: Investigation of glucose-6-phosphate dehydrogenase (G6PD) deficiency prevalence in a Plasmodium vivax -endemic area in the Republic of Korea (ROK)

doi: 10.1186/s12936-020-03393-4

Figure Lengend Snippet: Screening results from seven representative G6PD variants using the DiaPlexC G6PD genotyping Kit (Asian type). To detect 7 different G6PD variants, including Vanua Lava (383 T>C), Mahidol (487 G>A), Coimbra (592 C>T), Viangchan (871 G>A), Union (1360 C>T), Canton (1376 G>T), and Kalping (1388 G>A), 170 blood samples representing different ranges of G6PD activity level [n = 131 samples with partially low normal, 30–80% (2.27–6.05 U/g Hb) of the median; n = 3 samples with high, > 150% (> 11.373 U/g Hb) of the median; and n = 36 samples with normal, 80–150% (6.05–11.373 U/g Hb) of the median] (Among 170 samples, 82 samples were collected from Paju and 88 samples from Yangju) were screened with the one-step PCR method of the DiaPlexC kit. IC: internal control, WC: wild-type control, MC: mutant-type control

Article Snippet: Fig. 2 Comparison of two analytical methods for representing G6PD activity: Point Scientific G-6-PDH kit and CareStart G6PD Biosensor.

Techniques: Activity Assay, Control, Mutagenesis

Journal: Malaria Journal

Article Title: Investigation of glucose-6-phosphate dehydrogenase (G6PD) deficiency prevalence in a Plasmodium vivax -endemic area in the Republic of Korea (ROK)

doi: 10.1186/s12936-020-03393-4

Figure Lengend Snippet: Profiles of eight participants with mutations. Basic information (region, age, gender, and G6PD activity) of participants with a C1311T exon mutation or a T93C intron mutation

Article Snippet: Fig. 2 Comparison of two analytical methods for representing G6PD activity: Point Scientific G-6-PDH kit and CareStart G6PD Biosensor.

Techniques: Activity Assay, Mutagenesis